Reference/LiquidCulture/Recipes/PGT
From Pikes Peak Myco Wiki
This recipe comes from Philly Golden Teacher on YouTube.[1] It's a reliable, widely used starting point.
| Ingredient | Amount | Notes |
|---|---|---|
| Water | 600 ml | Distilled gives the clearest result |
| Karo light corn syrup | 18–20 g | The main sugar, roughly 3% of the water by weight |
| Light dry malt extract (LME) | 1.5 g | |
| Peptone | 1 g | Nutritional yeast works as a substitute |
Directions
- Dissolve everything in warm water and divide between jars fitted with an injection port and a filter (see making and using LC). Add a stir bar to each jar if you have a magnetic stirrer.
- Pressure cook at 15 psi for 30 minutes at sea level.
- At about 6,000 ft, cook 45–60 minutes at 15 psi, or raise the pressure by about 3 psi if your cooker has a dial gauge. Most hobby cookers use a fixed 15 psi weight, so extending the time is the usual choice. See pressure cooking at altitude for why.
- Let the cooker cool and depressurize on its own, and let the jars reach room temperature before inoculating.
Notes
- Compared with the ClemGrows recipe, this one is somewhat richer. Both work; ClemGrows trades a little nutrition for clarity and slower blob formation.
- If the liquid comes out dark amber, it cooked too long or too hot. A light gold tint is normal.
References
- ↑ Philly Golden Teacher, liquid culture video (YouTube).
| Cultivation guide | |
|---|---|
| Basics | Sterile technique · Pressure cooking at altitude · Glossary · Equipment |
| Culture work | Spores · Agar plates · Three transfers · Cloning · Storing cultures · Smash tek · Liquid culture · LC recipes: PGT, ClemGrows |
| Spawn & substrate | Grain spawn · Coir and CVG · Manure-based substrates |
| Fruiting & problems | Monotub fruiting · Contamination ID · Drying and storage |
