https://wiki.pikespeakmycoleague.org/index.php?title=Special:RecentChanges Track the most recent changes to the wiki in this feed. en MediaWiki 1.46.0 Sat, 24 Oct 2026 14:07:02 GMT https://wiki.pikespeakmycoleague.org/index.php?title=MediaWiki:Sidebar&diff=117&oldid=116 https://wiki.pikespeakmycoleague.org/index.php?title=MediaWiki:Sidebar&diff=117&oldid=116 <p></p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:47, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l5">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class="diff-marker"></td><td 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0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>** helppage|help-mediawiki</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>** library|<del style="font-weight: bold; text-decoration: none;">library</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>** library|<ins style="font-weight: bold; text-decoration: none;">Library</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>** specialpages-url|specialpages</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>** specialpages-url|specialpages</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* SEARCH</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* SEARCH</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* TOOLBOX</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* TOOLBOX</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* LANGUAGES</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* LANGUAGES</div></td></tr> </table> Thu, 22 Oct 2026 21:47:30 GMT Cbenesch https://wiki.pikespeakmycoleague.org/index.php?title=MediaWiki_talk:Sidebar https://wiki.pikespeakmycoleague.org/index.php?title=MediaWiki:Sidebar&diff=116&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=MediaWiki:Sidebar&diff=116&oldid=0 <p>Created page with &quot; * navigation ** mainpage|mainpage-description ** recentchanges-url|recentchanges ** randompage-url|randompage ** helppage|help-mediawiki ** library|library ** specialpages-url|specialpages * SEARCH * TOOLBOX * LANGUAGES&quot;</p> <p><b>New page</b></p><div><br /> * navigation<br /> ** mainpage|mainpage-description<br /> ** recentchanges-url|recentchanges<br /> ** randompage-url|randompage<br /> ** helppage|help-mediawiki<br /> ** library|library<br /> ** specialpages-url|specialpages<br /> * SEARCH<br /> * TOOLBOX<br /> * LANGUAGES</div> Thu, 22 Oct 2026 21:46:56 GMT Cbenesch https://wiki.pikespeakmycoleague.org/index.php?title=MediaWiki_talk:Sidebar https://wiki.pikespeakmycoleague.org/index.php?title=Library&diff=115&oldid=114 https://wiki.pikespeakmycoleague.org/index.php?title=Library&diff=115&oldid=114 <p></p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 21:44, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">&lt;nowiki&gt;</del>&lt;dpl&gt; namespace = File titlematch = %.pdf mode = gallery &lt;/dpl<del style="font-weight: bold; text-decoration: none;">&gt;&lt;/nowiki</del>&gt;</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>&lt;dpl&gt;</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>namespace = File</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>titlematch = %.pdf</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>mode = gallery</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>&lt;/dpl&gt;</div></td></tr> </table> Thu, 22 Oct 2026 21:44:03 GMT Cbenesch https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Library https://wiki.pikespeakmycoleague.org/index.php?title=Library&diff=114&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Library&diff=114&oldid=0 <p>Created page with &quot;&lt;nowiki&gt;&lt;dpl&gt; namespace = File titlematch = %.pdf mode = gallery &lt;/dpl&gt;&lt;/nowiki&gt;&quot;</p> <p><b>New page</b></p><div>&lt;nowiki&gt;&lt;dpl&gt; namespace = File titlematch = %.pdf mode = gallery &lt;/dpl&gt;&lt;/nowiki&gt;</div> Thu, 22 Oct 2026 21:42:37 GMT Cbenesch https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Library https://wiki.pikespeakmycoleague.org/index.php?title=File:The-psilocybin-mushroom-bible_compress.pdf&diff=113&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:The-psilocybin-mushroom-bible_compress.pdf&diff=113&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:The-psilocybin-mushroom-bible_compress.pdf" title="File:The-psilocybin-mushroom-bible compress.pdf">File:The-psilocybin-mushroom-bible compress.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:34:01 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:The-psilocybin-mushroom-bible_compress.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:The-psilocybin-chef-cookbook-compressed.pdf&diff=112&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:The-psilocybin-chef-cookbook-compressed.pdf&diff=112&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:The-psilocybin-chef-cookbook-compressed.pdf" title="File:The-psilocybin-chef-cookbook-compressed.pdf">File:The-psilocybin-chef-cookbook-compressed.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:34:00 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:The-psilocybin-chef-cookbook-compressed.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Mushrooms.pdf&diff=111&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Mushrooms.pdf&diff=111&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Mushrooms.pdf" title="File:Mushrooms.pdf">File:Mushrooms.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:34:00 GMT Maintenance script 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https://wiki.pikespeakmycoleague.org/index.php?title=File:Volvariella_growing.pdf_%C2%B7_version_1.pdf&diff=107&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Volvariella_growing.pdf_%C2%B7_version_1.pdf" title="File:Volvariella growing.pdf · version 1.pdf">File:Volvariella growing.pdf · version 1.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:34:00 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Volvariella_growing.pdf_%C2%B7_version_1.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Technologies_for_Mushroom_Production.pdf&diff=106&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Technologies_for_Mushroom_Production.pdf&diff=106&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Technologies_for_Mushroom_Production.pdf" title="File:Technologies for Mushroom Production.pdf">File:Technologies for Mushroom Production.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:34:00 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Technologies_for_Mushroom_Production.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Psilocybin_Mushrooms_by_David_Nature.pdf&diff=105&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Psilocybin_Mushrooms_by_David_Nature.pdf&diff=105&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Psilocybin_Mushrooms_by_David_Nature.pdf" title="File:Psilocybin Mushrooms by David Nature.pdf">File:Psilocybin Mushrooms by David Nature.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:33:59 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Psilocybin_Mushrooms_by_David_Nature.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Psilocybin_Mushroom_Handbook_G_Nicholas.pdf&diff=104&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Psilocybin_Mushroom_Handbook_G_Nicholas.pdf&diff=104&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Psilocybin_Mushroom_Handbook_G_Nicholas.pdf" title="File:Psilocybin Mushroom Handbook G Nicholas.pdf">File:Psilocybin Mushroom Handbook G Nicholas.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:33:59 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Psilocybin_Mushroom_Handbook_G_Nicholas.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Oyster_Mushroom_Cultivation.pdf&diff=103&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Oyster_Mushroom_Cultivation.pdf&diff=103&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Oyster_Mushroom_Cultivation.pdf" title="File:Oyster Mushroom Cultivation.pdf">File:Oyster Mushroom Cultivation.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:33:59 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Oyster_Mushroom_Cultivation.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Mushroom_Growers_Handbook_1_-_Oyster_Mushroom_Cultivation.pdf&diff=102&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Mushroom_Growers_Handbook_1_-_Oyster_Mushroom_Cultivation.pdf&diff=102&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Mushroom_Growers_Handbook_1_-_Oyster_Mushroom_Cultivation.pdf" title="File:Mushroom Growers Handbook 1 - Oyster Mushroom Cultivation.pdf">File:Mushroom Growers Handbook 1 - Oyster Mushroom Cultivation.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:33:59 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Mushroom_Growers_Handbook_1_-_Oyster_Mushroom_Cultivation.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Mushroom-Tree-Compatability_v3.pdf_%C2%B7_version_1.pdf&diff=101&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Mushroom-Tree-Compatability_v3.pdf_%C2%B7_version_1.pdf&diff=101&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Mushroom-Tree-Compatability_v3.pdf_%C2%B7_version_1.pdf" title="File:Mushroom-Tree-Compatability v3.pdf · version 1.pdf">File:Mushroom-Tree-Compatability v3.pdf · version 1.pdf</a> == Summary == Importing file</p> <p><b>New 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Thu, 22 Oct 2026 20:33:58 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Mushroom_Growing_Instructions.pdf_%C2%B7_version_1.pdf https://wiki.pikespeakmycoleague.org/index.php?title=File:Medicinal_Mushrooms-_Their_Therapeutic_Properties_and_Current_Medical_Usage_with_Special_Emphasis_on_Cancer_Treatments.pdf&diff=99&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=File:Medicinal_Mushrooms-_Their_Therapeutic_Properties_and_Current_Medical_Usage_with_Special_Emphasis_on_Cancer_Treatments.pdf&diff=99&oldid=0 <p><a href="/index.php?title=User:Maintenance_script&amp;action=edit&amp;redlink=1" class="mw-userlink new" title="User:Maintenance script (page does not exist)"><bdi>Maintenance script</bdi></a> uploaded <a href="/index.php?title=File:Medicinal_Mushrooms-_Their_Therapeutic_Properties_and_Current_Medical_Usage_with_Special_Emphasis_on_Cancer_Treatments.pdf" title="File:Medicinal Mushrooms- Their Therapeutic Properties and Current 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href="/index.php?title=File:Low_Cost_Mushroom_Cultivation.pdf_%C2%B7_version_1.pdf" title="File:Low Cost Mushroom Cultivation.pdf · version 1.pdf">File:Low Cost Mushroom Cultivation.pdf · version 1.pdf</a> == Summary == Importing file</p> <p><b>New page</b></p><div>== Summary ==<br /> Importing file</div> Thu, 22 Oct 2026 20:33:58 GMT Maintenance script https://wiki.pikespeakmycoleague.org/index.php?title=File_talk:Low_Cost_Mushroom_Cultivation.pdf_%C2%B7_version_1.pdf https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Spawn/Grain_Spawn&diff=57&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Spawn/Grain_Spawn&diff=57&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>&#039;&#039;&#039;Spawn&#039;&#039;&#039; is a nutritious material, usually grain, that has been fully colonized by mycelium. It&#039;s the engine of a grow: you mix it into bulk substrate, and the mycelium spreads from every kernel at once.<br /> <br /> Grain needs to be &#039;&#039;&#039;sterilized&#039;&#039;&#039;, not just pasteurized. It&#039;s so nutritious that anything left alive in it will take over.<br /> <br /> == Choosing a grain ==<br /> {| class=&quot;wikitable&quot;<br /> ! Grain !! Pros !! Cons<br /> |-<br /> | &#039;&#039;&#039;Whole oats&#039;&#039;&#039; || Cheap, easy to find (feed stores sell 50 lb bags), forgiving || Hulls can trap moisture<br /> |-<br /> | &#039;&#039;&#039;Rye berries&#039;&#039;&#039; || The classic. Holds water well, big kernels || Pricier, harder to find, easy to over-hydrate<br /> |-<br /> | &#039;&#039;&#039;Millet&#039;&#039;&#039; || Many small kernels mean many inoculation points and fast colonization || Small kernels clump if too wet<br /> |-<br /> | &#039;&#039;&#039;Wild bird seed (WBS)&#039;&#039;&#039; || Very cheap mixed seed || Quality varies; pick a mix without sunflower seeds or added oils<br /> |-<br /> | &#039;&#039;&#039;Brown rice / popcorn&#039;&#039;&#039; || Available at any grocery store || Rice gets mushy easily; popcorn has a hard hull<br /> |}<br /> <br /> == Preparation ==<br /> === 1. Rinse ===<br /> Rinse the grain in a bucket of water, stirring and dumping until the water runs mostly clear. This removes dust, chaff, and floating debris.<br /> <br /> === 2. Soak (12–24 hours) ===<br /> Cover with plenty of water and leave it at room temperature overnight. Besides hydrating the grain, soaking wakes up heat-resistant bacterial spores (endospores) so the pressure cooker can kill them. Skipping the soak is a common cause of [[Reference/Contamination#Bacteria|bacterial contamination]].<br /> <br /> === 3. Simmer (10–15 minutes) ===<br /> Drain, cover with fresh water, and simmer until the kernels are swollen and you can squish one between your fingers, but few are split open. Split kernels release starch and make sticky, bacteria-friendly clumps.<br /> <br /> === 4. Dry the surface ===<br /> Drain and spread the grain on a towel or screen for 30–60 minutes, until the outside of each kernel looks dry but the inside is plump. This is the most important step. &#039;&#039;&#039;Wet grain is the number one cause of failed grain jars.&#039;&#039;&#039;<br /> <br /> A small amount of gypsum mixed in now helps keep kernels separate. It&#039;s optional.<br /> <br /> === 5. Load the jars ===<br /> Fill jars or bags about two-thirds to three-quarters full so there&#039;s room to shake. Use lids with an [[Tutorials/LiquidCulture/Making and Using LC#Equipment|injection port and filter]]. Cover the lids with foil.<br /> <br /> === 6. Pressure cook ===<br /> {| class=&quot;wikitable&quot;<br /> ! Container !! At sea level (15 psi) !! Front Range (15 psi weight)<br /> |-<br /> | Quart jars || 90 min || 120–150 min<br /> |-<br /> | Grain bags, 3–5 lb || 2.5 hr || 3–3.5 hr<br /> |}<br /> See [[Reference/Pressure Cooking at Altitude|pressure cooking at altitude]] for the reasoning. Let the cooker depressurize on its own, then let the jars cool to room temperature, ideally overnight.<br /> <br /> == Checking hydration ==<br /> After cooking there should be &#039;&#039;&#039;no standing water&#039;&#039;&#039; in the bottom of the jar. Kernels should be plump and separate, not stuck together in a mass. If you see water pooled at the bottom, that jar has a much higher risk of bacteria.<br /> <br /> == Inoculating ==<br /> In a [[Tutorials/Basics/Sterile Technique|still air box or flow hood]]:<br /> * &#039;&#039;&#039;Liquid culture:&#039;&#039;&#039; 1–3 ml per quart jar, 5–10 ml per bag.<br /> * &#039;&#039;&#039;Agar:&#039;&#039;&#039; 2–4 wedges per quart jar.<br /> * &#039;&#039;&#039;Spores:&#039;&#039;&#039; 2–4 ml per quart jar. Spore-started jars are slower and riskier than LC or agar.<br /> <br /> == Incubating ==<br /> * Keep jars at 70–80°F, in the dark. Darkness isn&#039;t critical, but light can encourage early pinning in the jar.<br /> * You&#039;ll see the first white growth around the inoculation points in about 3–10 days.<br /> * &#039;&#039;&#039;Shake&#039;&#039;&#039; when the jar is about 20–30% colonized: break up the grain and spread the colonized kernels throughout. This dramatically speeds up colonization. Some growers shake once more around 60%.<br /> * A jar is ready when every kernel is wrapped in white. Give it a few more days after it looks finished so the mycelium consolidates.<br /> <br /> == Grain to grain (G2G) ==<br /> A fully colonized jar can inoculate more sterilized grain. Roughly 1 colonized jar to 10 fresh jars works well. This stretches one good culture into a lot of spawn, but every transfer is a chance to spread contamination, so only do it from jars that are clearly clean.<br /> <br /> == What&#039;s next ==<br /> Spawn goes into bulk substrate: [[Tutorials/Substrate/Coir and CVG|coir and CVG]] or [[Tutorials/Substrate/Manure-Based Substrates|manure-based substrates]], then on to [[Tutorials/Fruiting/Monotub|fruiting]].<br /> <br /> {{Cultivation}}<br /> [[Category:Tutorials]]<br /> [[Category:Spawn]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/Spawn/Grain_Spawn https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/LiquidCulture/Making_and_Using_LC&diff=56&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/LiquidCulture/Making_and_Using_LC&diff=56&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Liquid culture (LC) is mycelium grown in sugar water. A single jar can inoculate dozens of grain jars, and because the mycelium is already alive and growing, it gets established on grain much faster than spores do.<br /> <br /> The trade-off: contamination in liquid is hard to see. Always [[#Testing|test your LC]] before trusting it with a big batch of grain.<br /> <br /> == Equipment ==<br /> * &#039;&#039;&#039;Jars:&#039;&#039;&#039; pint or quart mason jars. Fill only about halfway so there&#039;s room for air.<br /> * &#039;&#039;&#039;Lids&#039;&#039;&#039; with a self-healing &#039;&#039;&#039;injection port&#039;&#039;&#039; (silicone or a rubber stopper) for adding and drawing culture, and a &#039;&#039;&#039;gas exchange filter&#039;&#039;&#039; (a synthetic filter disc or a hole covered with micropore tape).<br /> * &#039;&#039;&#039;Stir bar&#039;&#039;&#039; (optional but recommended): a small magnetic bar in each jar, plus a magnetic stir plate. Without one you can swirl by hand, or add a few glass marbles to help break up clumps.<br /> * &#039;&#039;&#039;Syringes&#039;&#039;&#039; with long needles (16–18 gauge is easier to draw up thick LC).<br /> <br /> == Recipes ==<br /> * [[Reference/LiquidCulture/Recipes/ClemGrows|ClemGrows LC]]: low-nutrient, crystal clear, resists blobbing<br /> * [[Reference/LiquidCulture/Recipes/PGT|Philly Golden Teacher LC]]: a bit richer, very forgiving<br /> <br /> Most good recipes land around 2–4% sugars by weight. More is not better: high-nutrient LC grows into thick mats on the surface, turns cloudy, and caramelizes in the cooker.<br /> <br /> == Making it ==<br /> # Mix the recipe and fill jars about halfway. Drop in stir bars.<br /> # Cover the filters with foil so condensation doesn&#039;t soak them.<br /> # [[Reference/Pressure Cooking at Altitude|Pressure cook]], adjusting time for altitude.<br /> # Let the jars cool completely before inoculating. Heat will kill your culture.<br /> <br /> == Inoculating ==<br /> Working in a [[Tutorials/Basics/Sterile Technique|still air box or flow hood]]:<br /> * &#039;&#039;&#039;From agar (best):&#039;&#039;&#039; cut a few small wedges from a clean plate and drop them in through a briefly opened lid, or push them through a wide port.<br /> * &#039;&#039;&#039;From a spore syringe:&#039;&#039;&#039; inject 1–2 ml through the port. Spore-started LC can&#039;t be checked for contamination until it&#039;s grown, so testing it is especially important.<br /> * &#039;&#039;&#039;From LC:&#039;&#039;&#039; 1 ml of good LC starts a new jar.<br /> <br /> Wipe the port with alcohol before every needle goes in, and flame the needle until it glows red, then let it cool.<br /> <br /> == Growing it ==<br /> * Keep jars at room temperature, around 70–78°F, out of direct sun.<br /> * Stir or swirl gently once a day or every few days to break up clumps and spread the mycelium.<br /> * Healthy LC shows white clouds, wisps, or clumps of mycelium in clear or lightly tinted liquid. Most jars are ready in 1–3 weeks.<br /> <br /> === Signs of trouble ===<br /> {| class=&quot;wikitable&quot;<br /> ! What you see !! Likely cause<br /> |-<br /> | The whole liquid turns uniformly cloudy, milky, or murky within days || Bacteria<br /> |-<br /> | A slimy film on the surface or a sour smell || Bacteria<br /> |-<br /> | Green, blue, black, or colored fuzz || Mold. See [[Reference/Contamination|contamination ID]]<br /> |-<br /> | Dark amber before you ever inoculated || Overcooked. Usable, but not ideal.<br /> |}<br /> <br /> Clear liquid with white mycelium is what you&#039;re after. Cloudiness that clears when the jar settles may just be stirred-up mycelium; cloudiness that stays is a red flag.<br /> <br /> == Testing ==<br /> Before inoculating a big run of grain, put a drop of LC on an [[Tutorials/Agar/Making Plates|agar plate]] and on one test grain jar. If both grow clean white mycelium within a week or so, use the rest with confidence. This step catches most bad LC before it ruins a whole batch.<br /> <br /> == Using and storing it ==<br /> * Shake or stir the jar first so you draw up mycelium, not just liquid.<br /> * Typical inoculation is 1–3 ml per quart jar of grain, or 5–10 ml per grain bag.<br /> * Store finished LC in the fridge. It keeps for several months; let it warm up and give it a stir before use.<br /> <br /> {{Cultivation}}<br /> [[Category:Tutorials]]<br /> [[Category:Liquid culture]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/LiquidCulture/Making_and_Using_LC https://wiki.pikespeakmycoleague.org/index.php?title=Reference/LiquidCulture/Recipes/ClemGrows&diff=55&oldid=44 https://wiki.pikespeakmycoleague.org/index.php?title=Reference/LiquidCulture/Recipes/ClemGrows&diff=55&oldid=44 <p>Wiki rebuild: cleanup and new cultivation articles</p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:00, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Ok y</del>'<del style="font-weight: bold; text-decoration: none;">all </del>here<del style="font-weight: bold; text-decoration: none;">'s my lecture </del>for the <del style="font-weight: bold; text-decoration: none;">day  I</del>'<del style="font-weight: bold; text-decoration: none;">m sure most of y</del>'<del style="font-weight: bold; text-decoration: none;">all have heard me Nerd the fuck out about Liquid Culture.</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>'<ins style="font-weight: bold; text-decoration: none;">'Written by MDot (ClemGrows), a former league admin, and shared </ins>here <ins style="font-weight: bold; text-decoration: none;">with his permission. Lightly edited </ins>for the <ins style="font-weight: bold; text-decoration: none;">wiki.</ins>''</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* 300 ml distilled water</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[File:ClemGrowsLC</ins>.<ins style="font-weight: bold; text-decoration: none;">png|thumb|right|The original recipe card from ClemGrows]]</ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* 1/64 (very small amount) Light Malt Extract</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* 1/4 tsp</del>. <del style="font-weight: bold; text-decoration: none;">Peptone (or nutritional yeast)</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* 1 </del>tsp Karo corn syrup</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== The recipe ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{| class="wikitable"</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">! Ingredient !! Amount !! Notes</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|-</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| Distilled water, hot || 300 ml ||</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|-</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| Peptone || ¼ </ins>tsp <ins style="font-weight: bold; text-decoration: none;">|| Nutritional yeast works as a substitute</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|-</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| </ins>Karo <ins style="font-weight: bold; text-decoration: none;">light </ins>corn syrup <ins style="font-weight: bold; text-decoration: none;">|| 1 tsp ||</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|-</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| Light malt extract (LME) || 1/64 tsp || A tiny amount. Novelty measuring-spoon sets label this size "drop."</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|}</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>30 <del style="font-weight: bold; text-decoration: none;">min pressure cooker sanitization</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">'''Pressure cook </ins>30 <ins style="font-weight: bold; text-decoration: none;">minutes''' at 15 psi. At Front Range elevations, see the warning below about cooking longer.</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">About </del>how important I <del style="font-weight: bold; text-decoration: none;">believe </del>it is to be dialed <del style="font-weight: bold; text-decoration: none;">af. How I believe my recipe is as good as it gets and the amount of </del>work <del style="font-weight: bold; text-decoration: none;">I've put </del>into getting it <del style="font-weight: bold; text-decoration: none;">dialed like this. Why </del>I <del style="font-weight: bold; text-decoration: none;">believe </del>it's <del style="font-weight: bold; text-decoration: none;">so important. I'd just like to show y'all and talk about </del>it<del style="font-weight: bold; text-decoration: none;">. Id also like to show you how close it is to teetering on the edge of perfection</del>.  </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Why this recipe ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Ok y'all, here's my lecture for the day. I'm sure most of you have heard me nerd out about liquid culture: </ins>how important I <ins style="font-weight: bold; text-decoration: none;">think </ins>it is to be dialed <ins style="font-weight: bold; text-decoration: none;">in, how much </ins>work <ins style="font-weight: bold; text-decoration: none;">went </ins>into getting <ins style="font-weight: bold; text-decoration: none;">this recipe where </ins>it <ins style="font-weight: bold; text-decoration: none;">is, and why </ins>I <ins style="font-weight: bold; text-decoration: none;">think </ins>it's <ins style="font-weight: bold; text-decoration: none;">as good as </ins>it <ins style="font-weight: bold; text-decoration: none;">gets</ins>.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">I have my recipe pinned in this channel so everyone has access to a reliable recipe. </del>The recipes on the internet <del style="font-weight: bold; text-decoration: none;">are shitty af. Will they </del>work<del style="font-weight: bold; text-decoration: none;">? Yes. But </del>if you <del style="font-weight: bold; text-decoration: none;">are gunna do it</del>, why not do it great? The problem with almost all <del style="font-weight: bold; text-decoration: none;">the recipes out there </del>is <del style="font-weight: bold; text-decoration: none;">they are way </del>too <del style="font-weight: bold; text-decoration: none;">high </del>nutrient. You don't need that. You actually don't want <del style="font-weight: bold; text-decoration: none;">that</del>. All <del style="font-weight: bold; text-decoration: none;">that </del>does is make your culture lazy <del style="font-weight: bold; text-decoration: none;">af </del>and slow <del style="font-weight: bold; text-decoration: none;">and </del>it overgrows the surface of the liquid and turns into <del style="font-weight: bold; text-decoration: none;">a shitty </del>unusable <del style="font-weight: bold; text-decoration: none;">like </del>blob <del style="font-weight: bold; text-decoration: none;">fast</del>. It does not make the jar colonize faster.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The recipes on the internet <ins style="font-weight: bold; text-decoration: none;">will </ins>work<ins style="font-weight: bold; text-decoration: none;">, but </ins>if you<ins style="font-weight: bold; text-decoration: none;">'re going to make LC</ins>, why not do it great? The problem with almost all <ins style="font-weight: bold; text-decoration: none;">of them </ins>is too <ins style="font-weight: bold; text-decoration: none;">much </ins>nutrient. You don't need that. You actually don't want <ins style="font-weight: bold; text-decoration: none;">it</ins>. All <ins style="font-weight: bold; text-decoration: none;">it </ins>does is make your culture lazy and slow<ins style="font-weight: bold; text-decoration: none;">; </ins>it overgrows the surface of the liquid and <ins style="font-weight: bold; text-decoration: none;">quickly </ins>turns into <ins style="font-weight: bold; text-decoration: none;">an </ins>unusable blob. It does not make the jar colonize <ins style="font-weight: bold; text-decoration: none;">any </ins>faster.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">With my </del>recipe <del style="font-weight: bold; text-decoration: none;">you will see in the pictures what I love about it. It is the exact right amount of </del>nutrients. It colonizes fast and <del style="font-weight: bold; text-decoration: none;">ridiculously </del>thick<del style="font-weight: bold; text-decoration: none;">. But </del>once the jar is almost full, instead of <del style="font-weight: bold; text-decoration: none;">just growing </del>and growing <del style="font-weight: bold; text-decoration: none;">and eating </del>and eating, it basically runs out of <del style="font-weight: bold; text-decoration: none;">nutrients</del>. Not completely but enough <del style="font-weight: bold; text-decoration: none;">that it puts </del>the brakes on <del style="font-weight: bold; text-decoration: none;">the growth</del>. <del style="font-weight: bold; text-decoration: none;">You'll see in </del>the pictures it <del style="font-weight: bold; text-decoration: none;">colonizes </del>beautifully right up until it's about to reach the surface, then <del style="font-weight: bold; text-decoration: none;">it stops. This keeps your LC hungry, aggressive, ready for grain. It also keeps it much higher quality. If anyone has done LC and didn't stir it for awhile you know just how shitty it can get quick. Mine still needs stirred, but about 4x less often as normal recipes . Once it forms Into a big blob up top it's hard af to break up, even if you do get it broke up it looks shitty in the syringe bc you can see the myc  is different. And when it does that it's very very hard to suck up a needle</del>.  </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== It stops itself ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">This </ins>recipe <ins style="font-weight: bold; text-decoration: none;">has exactly enough </ins>nutrients. It colonizes fast and thick<ins style="font-weight: bold; text-decoration: none;">, but </ins>once the jar is almost full, instead of <ins style="font-weight: bold; text-decoration: none;">eating </ins>and growing and eating, it basically runs out of <ins style="font-weight: bold; text-decoration: none;">food</ins>. Not completely<ins style="font-weight: bold; text-decoration: none;">, </ins>but enough <ins style="font-weight: bold; text-decoration: none;">to put </ins>the brakes on. <ins style="font-weight: bold; text-decoration: none;">In </ins>the pictures <ins style="font-weight: bold; text-decoration: none;">you can see </ins>it <ins style="font-weight: bold; text-decoration: none;">colonize </ins>beautifully right up until it's about to reach the surface, then <ins style="font-weight: bold; text-decoration: none;">stop</ins>.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Using </del>the <del style="font-weight: bold; text-decoration: none;">correct ratios and recipe is also how </del>you <del style="font-weight: bold; text-decoration: none;">achieve perfectly clear </del>LC. This <del style="font-weight: bold; text-decoration: none;">recipe </del>LC <del style="font-weight: bold; text-decoration: none;">is literally better in every single way. If </del>you <del style="font-weight: bold; text-decoration: none;">have received syringes from me </del>you <del style="font-weight: bold; text-decoration: none;">know how full they </del>can <del style="font-weight: bold; text-decoration: none;">be, without going crazy high nute</del>. <del style="font-weight: bold; text-decoration: none;">And it takes off on grain almost immediately</del>.  </div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">That keeps your LC hungry, aggressive, and ready for grain, and it keeps </ins>the <ins style="font-weight: bold; text-decoration: none;">quality much higher. If </ins>you<ins style="font-weight: bold; text-decoration: none;">'ve ever left an </ins>LC <ins style="font-weight: bold; text-decoration: none;">unstirred for a while, you know how fast it gets ugly</ins>. This <ins style="font-weight: bold; text-decoration: none;">one still needs stirring, but about a quarter as often as typical recipes. Once </ins>LC <ins style="font-weight: bold; text-decoration: none;">forms a big blob on top it's hard to break up, and even when </ins>you <ins style="font-weight: bold; text-decoration: none;">do, </ins>you can <ins style="font-weight: bold; text-decoration: none;">see in the syringe that the mycelium looks different</ins>. <ins style="font-weight: bold; text-decoration: none;">It's also very hard to draw up a needle</ins>.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">The other thing I want to show y</del>'<del style="font-weight: bold; text-decoration: none;">all is </del>how <del style="font-weight: bold; text-decoration: none;">much of </del>an art <del style="font-weight: bold; text-decoration: none;">this is. </del>Same as <del style="font-weight: bold; text-decoration: none;">making </del>agar, the smallest <del style="font-weight: bold; text-decoration: none;">of </del>changes make a massive difference. <del style="font-weight: bold; text-decoration: none;">You'll see in these pictures, super clear perfect LC, then you'll see a dark gold not nearly as good looking jar. Both are same </del>recipe<del style="font-weight: bold; text-decoration: none;">. The thing with the recipe is it's dialed, it's </del>at the peak<del style="font-weight: bold; text-decoration: none;">. So it's the exact amount of nutes you need for </del>a <del style="font-weight: bold; text-decoration: none;">full thick jar, while also keeping the LC Crystal clear, not hazy, zero sediment. So if you add just the tiniest amount </del>too much it <del style="font-weight: bold; text-decoration: none;">will begin to decline from the peak. </del>1/<del style="font-weight: bold; text-decoration: none;">32nd </del>tsp too much LME <del style="font-weight: bold; text-decoration: none;">and it</del>'<del style="font-weight: bold; text-decoration: none;">s now </del>hazy <del style="font-weight: bold; text-decoration: none;">and has </del>sediment<del style="font-weight: bold; text-decoration: none;">. </del>1/<del style="font-weight: bold; text-decoration: none;">8th </del>tsp too much <del style="font-weight: bold; text-decoration: none;">Peptone and it</del>'<del style="font-weight: bold; text-decoration: none;">s </del>amber and hazy<del style="font-weight: bold; text-decoration: none;">. 1/4tsp </del>too much <del style="font-weight: bold; text-decoration: none;">karo and </del>it caramelizes <del style="font-weight: bold; text-decoration: none;">like a MF. Pressure cooking for </del>5 <del style="font-weight: bold; text-decoration: none;">mins </del>too long <del style="font-weight: bold; text-decoration: none;">and </del>it caramelizes<del style="font-weight: bold; text-decoration: none;">. The measurements in the recipe are super important. </del>There are ways <del style="font-weight: bold; text-decoration: none;">you can </del>add a <del style="font-weight: bold; text-decoration: none;">very small amount </del>more of each but it's difficult to get right.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== Clear, not cloudy ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Getting the ratios right is also how you get perfectly clear LC: no haze, zero sediment. If you</ins>'<ins style="font-weight: bold; text-decoration: none;">ve received syringes from me, you know </ins>how <ins style="font-weight: bold; text-decoration: none;">full they get without going high-nutrient. And it takes off on grain almost immediately.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== It's </ins>an art <ins style="font-weight: bold; text-decoration: none;">===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Same as agar, the smallest changes make a massive difference. <ins style="font-weight: bold; text-decoration: none;">This </ins>recipe <ins style="font-weight: bold; text-decoration: none;">sits right </ins>at the peak<ins style="font-weight: bold; text-decoration: none;">, so adding even </ins>a <ins style="font-weight: bold; text-decoration: none;">tiny bit </ins>too much <ins style="font-weight: bold; text-decoration: none;">of anything starts </ins>it <ins style="font-weight: bold; text-decoration: none;">sliding downhill:</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''</ins>1/<ins style="font-weight: bold; text-decoration: none;">32 </ins>tsp too much LME<ins style="font-weight: bold; text-decoration: none;">:''</ins>' hazy<ins style="font-weight: bold; text-decoration: none;">, with </ins>sediment</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''</ins>1/<ins style="font-weight: bold; text-decoration: none;">8 </ins>tsp too much <ins style="font-weight: bold; text-decoration: none;">peptone:''</ins>' amber and hazy</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''¼ tsp </ins>too much <ins style="font-weight: bold; text-decoration: none;">Karo:''' </ins>it caramelizes <ins style="font-weight: bold; text-decoration: none;">hard</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''</ins>5 <ins style="font-weight: bold; text-decoration: none;">minutes </ins>too long <ins style="font-weight: bold; text-decoration: none;">in the pressure cooker:''' </ins>it caramelizes</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>There are ways <ins style="font-weight: bold; text-decoration: none;">to </ins>add a <ins style="font-weight: bold; text-decoration: none;">tiny bit </ins>more of each<ins style="font-weight: bold; text-decoration: none;">, </ins>but it's difficult to get right. <ins style="font-weight: bold; text-decoration: none;">The measurements matter.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">I'm all about the "Pursuit of Perfection," so I wanted to give a full breakdown of LC, why I think it matters, and how to get it right. If you're going to make LC, make the best LC you can.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Notes for high altitude ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">''Added by the wiki editors.''</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Because this recipe caramelizes with just a few extra minutes of cooking, it's a good one to watch at altitude. Sea-level guides say 30 minutes, but a 15 psi weight at 6,000 ft runs cooler (see [[Reference/Pressure Cooking at Altitude|pressure cooking at altitude]]). Two approaches:</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* If your cooker has a dial gauge, run it at about 18 psi for 30 minutes.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* With a fixed 15 psi weight, try 40–45 minutes and check the color. Crystal clear means it's right; amber means back the time off.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">For more on jars, inoculation, and stirring, see [[Tutorials/LiquidCulture/Making and Using LC|making and using LC]].</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{Cultivation}}</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Recipes]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Liquid culture]]</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">I nerd out about this and as y'all know, I'm all about the "Pursuit Of Perfection"  so I just wanted to give a full explanation and breakdown on LC, why I feel it's important and how to achieve it. If you are gunna make LC, why not make it the best LC you can! [[File:ClemGrowsLC.png|thumb|Liquid culture recipe from ClemGrows]]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> </table> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Reference/LiquidCulture/Recipes/ClemGrows https://wiki.pikespeakmycoleague.org/index.php?title=Reference/LiquidCulture/Recipes/PGT&diff=54&oldid=19 https://wiki.pikespeakmycoleague.org/index.php?title=Reference/LiquidCulture/Recipes/PGT&diff=54&oldid=19 <p>Wiki rebuild: cleanup and new cultivation articles</p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:00, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>This recipe <del style="font-weight: bold; text-decoration: none;">is </del>from Philly Golden Teacher on <del style="font-weight: bold; text-decoration: none;">Youtube and </del>a <del style="font-weight: bold; text-decoration: none;">perfectly viable and great recipe to use</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>This recipe <ins style="font-weight: bold; text-decoration: none;">comes </ins>from Philly Golden Teacher on <ins style="font-weight: bold; text-decoration: none;">YouTube. It's </ins>a <ins style="font-weight: bold; text-decoration: none;">reliable, widely used starting point.</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* </del>600 ml water</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{| class="wikitable"</ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* 18</del>-<del style="font-weight: bold; text-decoration: none;">20g Karo</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">! Ingredient !! Amount !! Notes</ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* </del>1.<del style="font-weight: bold; text-decoration: none;">5g Dry Light Malt Extract</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|-</ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* 1g </del>Peptone</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| Water || </ins>600 ml <ins style="font-weight: bold; text-decoration: none;">|| Distilled gives the clearest result</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|-</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| Karo light corn syrup || 18–20 g || The main sugar, roughly 3% of the </ins>water <ins style="font-weight: bold; text-decoration: none;">by weight</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|</ins>-</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| Light dry malt extract (LME) || </ins>1.<ins style="font-weight: bold; text-decoration: none;">5 g ||</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|-</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">| </ins>Peptone <ins style="font-weight: bold; text-decoration: none;">|| 1 g || Nutritional yeast works as a substitute</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">|}</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Pressure cook at <del style="font-weight: bold; text-decoration: none;">15psi </del>for 30 <del style="font-weight: bold; text-decoration: none;">min (45-60 </del>at <del style="font-weight: bold; text-decoration: none;">6000ft elevation</del>, or <del style="font-weight: bold; text-decoration: none;">increase </del>by <del style="font-weight: bold; text-decoration: none;">2-</del>3 psi.)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Directions ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># Dissolve everything in warm water and divide between jars fitted with an injection port and a filter (see [[Tutorials/LiquidCulture/Making and Using LC|making and using LC]]). Add a stir bar to each jar if you have a magnetic stirrer.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># </ins>Pressure cook at <ins style="font-weight: bold; text-decoration: none;">15 psi </ins>for 30 <ins style="font-weight: bold; text-decoration: none;">minutes at sea level.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># '''At about 6,000 ft, cook 45–60 minutes </ins>at <ins style="font-weight: bold; text-decoration: none;">15 psi</ins>, or <ins style="font-weight: bold; text-decoration: none;">raise the pressure </ins>by <ins style="font-weight: bold; text-decoration: none;">about </ins>3 psi<ins style="font-weight: bold; text-decoration: none;">''' if your cooker has a dial gauge. Most hobby cookers use a fixed 15 psi weight, so extending the time is the usual choice. See [[Reference/Pressure Cooking at Altitude|pressure cooking at altitude]] for why.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># Let the cooker cool and depressurize on its own, and let the jars reach room temperature before inoculating.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Notes ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Compared with the [[Reference/LiquidCulture/Recipes/ClemGrows|ClemGrows recipe]], this one is somewhat richer</ins>. <ins style="font-weight: bold; text-decoration: none;">Both work; ClemGrows trades a little nutrition for clarity and slower blob formation.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* If the liquid comes out dark amber, it cooked too long or too hot. A light gold tint is normal.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Sources ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Philly Golden Teacher, [https://www.youtube.com/watch?v=9XWVbkc--QE Liquid Culture video] (YouTube</ins>)</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{Cultivation}}</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Recipes]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Liquid culture]]</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[https://www.youtube.com/watch?v=9XWVbkc--QE Philly Golden Teacher Liquid Culture Video]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> </table> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Reference/LiquidCulture/Recipes/PGT https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Agar/Three-Transfers&diff=53&oldid=7 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Agar/Three-Transfers&diff=53&oldid=7 <p>Wiki rebuild: cleanup and new cultivation articles</p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:00, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>= Overview =</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>= Overview =</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">When working with agar plates, you'll often see them labeled with a T number (T1, T2, T3) and hear the advice to "transfer three times." This page explains what that means, why it works, and how to do it.</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">When working with agar plates, </del>a <del style="font-weight: bold; text-decoration: none;">lot of times we see them with </del>a <del style="font-weight: bold; text-decoration: none;">T number</del>, and <del style="font-weight: bold; text-decoration: none;">you may have even heard the term "transfer </del>three <del style="font-weight: bold; text-decoration: none;">times". This page will demonstrate and explain the concept and method of the "3 transfer" methodology</del>.</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">The short version: every time you move the best-looking piece of </ins>a <ins style="font-weight: bold; text-decoration: none;">culture to </ins>a <ins style="font-weight: bold; text-decoration: none;">fresh plate</ins>, <ins style="font-weight: bold; text-decoration: none;">you leave behind contamination </ins>and <ins style="font-weight: bold; text-decoration: none;">weaker genetics. After about </ins>three <ins style="font-weight: bold; text-decoration: none;">rounds, what's left is usually a single clean, fast-growing culture</ins>.</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>== Genetic <del style="font-weight: bold; text-decoration: none;">Selection </del>==</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[File:20260221 120037.jpg|thumb|right|300px|Plates at different transfer stages, each labeled with the strain, its transfer number, and the date.]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Why transfer at all ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== Cleaning up a culture ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Spore prints and swabs are never sterile. Spores, bacteria, and stray mold spores all come along for the ride. On agar they all grow, but they grow in ''different places'' on the plate. Mycelium outgrows most contamination at its leading edge, so a wedge from that edge is usually cleaner than the plate it came from. Each round leaves more of the passengers behind.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=</ins>== Genetic <ins style="font-weight: bold; text-decoration: none;">selection ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">A plate started from spores holds many genetically different individuals, each from a different spore. They grow side by side and often look visibly different: one sector fast and ropey, another fluffy and slow. These distinct regions are called '''sectors'''.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Fast colonization is one of the most valuable traits in any cultivated mushroom. A faster culture:</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Leaves contaminants less time to establish</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Shortens the whole cycle from inoculation to harvest</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Tends to produce stronger, more aggressive mycelium</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">By always picking from the fastest, healthiest sector, you're selecting for those traits.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== Rhizomorphic vs. tomentose ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''Rhizomorphic''' growth is ropey, with thick strands that radiate outward like roots. Growers generally favor it as a sign of vigorous genetics.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''Tomentose''' growth is fluffy and cottony, without clear strands. It isn't contamination, but it usually grows slower, and many growers avoid selecting it.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Healthy mycelium is normally white. Fluff that turns gray, green, or another color is [[Reference/Contamination|probably not yours]].</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== The method ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== T1: the starting plate ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Inoculate a plate from your spore syringe, swab, print, or tissue sample. Give it time to grow out. Expect it to look messy: several sectors, possibly some contamination at the inoculation point.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== T2: the first transfer ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># Find the healthiest-looking sector: fast, white, ideally rhizomorphic, and as far from any contamination as possible.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># Working in a [[Tutorials/Basics/Sterile Technique|still air box or flow hood]], flame your scalpel and let it cool.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># Cut a small wedge, about the size of a grain of rice, from the '''leading edge''' of that sector.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># Place it in the center of a fresh plate, mycelium side down.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># Label the new plate with the strain, '''T2''', and the date.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== T3: the second transfer ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Repeat the process from the T2 plate. By now growth should look much more even. Again take the leading edge of the best sector.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">=== Checking the result ===</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">A good T3 plate grows out evenly in every direction with one consistent look and no sectoring. If it still shows distinct sectors, keep going to T4 and beyond. "Three" is a rule of thumb, not a magic number. Some cultures are clean by T2; others need five or more rounds.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Tips ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''Transfer from the edge, not the middle.''' The center of the plate is the oldest growth, where the spores and contamination started.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''Label everything.''' Strain, transfer number, and date at minimum, as in the photo. Plates look identical in a stack.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''Run two or three plates per transfer.''' If one contaminates, you still have the others.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''Don't transfer too soon.''' Wait until the sector has clearly established itself and is growing away from the rest of the plate.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* '''Keep your winners.''' Once you have a clean, fast culture, store a few plates or slants in the fridge so you can start from it again later.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== What's next </ins>==</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">A clean plate can go straight to [[Tutorials/Spawn/Grain Spawn|grain]], or into [[Tutorials/LiquidCulture/Making and Using LC|liquid culture]] to expand it into many jars at once.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{Cultivation}}</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Tutorials]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">[[Category:Agar]]</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">One of the desirable genetics of any cultivable mushroom species is fast colonization. This leads to less risk of contamination of the growing medium, faster lifecycle from inoculation to harvest, and stronger mycelium.</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[[File:20260221 120037.jpg|thumb]]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> </table> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/Agar/Three-Transfers https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Agar/Making_Plates&diff=52&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Agar/Making_Plates&diff=52&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Agar is a gel made from seaweed. Mixed with a little sugar and nutrients, it gives mycelium a flat, clear surface to grow across, so you can actually &#039;&#039;see&#039;&#039; what&#039;s happening: whether a culture is clean, whether it&#039;s growing fast, and which part of it looks strongest. Agar is where you test, clean up, and select genetics before committing them to grain.<br /> <br /> == Recipes ==<br /> All recipes are per liter of water. Halve them for a smaller batch; 500 ml pours roughly 20–25 standard 100 mm plates.<br /> <br /> {| class=&quot;wikitable&quot;<br /> ! Recipe !! Agar powder !! Nutrients !! Notes<br /> |-<br /> | &#039;&#039;&#039;MEA&#039;&#039;&#039; (malt extract agar) || 20 g || 20–30 g light malt extract || The standard. Lab recipes use 30 g; many growers prefer 20 g.<br /> |-<br /> | &#039;&#039;&#039;Light MEA&#039;&#039;&#039; || 20 g || 10 g light malt extract || Lower nutrients make contamination easier to spot and push the culture to show its vigor.<br /> |-<br /> | &#039;&#039;&#039;MEA + yeast&#039;&#039;&#039; (MYA) || 20 g || 20 g light malt extract + 2 g nutritional yeast or peptone || Faster, denser growth. Slightly cloudier.<br /> |}<br /> <br /> Additions some growers use:<br /> * &#039;&#039;&#039;Distilled water&#039;&#039;&#039; gives the clearest plates. Colorado tap water is hard and can leave a haze.<br /> * A pinch of &#039;&#039;&#039;gypsum&#039;&#039;&#039; or a teaspoon of &#039;&#039;&#039;coir&#039;&#039;&#039; or ground grain helps the culture &quot;learn&quot; the substrate it will eventually eat.<br /> <br /> == Making the batch ==<br /> # Mix everything in a heat-safe bottle or jar with room to spare; a 1 L media bottle is ideal for 500 ml. Leave the cap loose or cover the mouth with foil.<br /> # Heat the mix in the microwave in short bursts, swirling between, until the agar powder fully dissolves and the liquid turns clear. Watch it closely; it boils over suddenly.<br /> # Pressure cook. At sea level that&#039;s 30 minutes at 15 psi; &#039;&#039;&#039;at Front Range elevations plan on 45–60 minutes&#039;&#039;&#039; (see [[Reference/Pressure Cooking at Altitude|pressure cooking at altitude]]).<br /> # Let the cooker depressurize naturally. Don&#039;t vent it early; the agar will boil out of the bottle.<br /> <br /> == Pouring ==<br /> # Set up in your [[Tutorials/Basics/Sterile Technique|still air box or flow hood]] with sleeves of plates ready.<br /> # Let the agar cool until you can hold the bottle bare-handed for a few seconds, around 120–140°F (50–60°C). Hotter agar makes more condensation; cooler agar starts setting in the bottle.<br /> # Lift each lid just enough to pour, fill the plate about one-third deep, and close it. Work in a stack so you only lift one lid at a time.<br /> # Leave plates to set for about an hour, then flip them upside down so condensation collects on the lid instead of dripping onto the agar.<br /> # Seal with Parafilm or plastic wrap, or keep them in the original sleeve.<br /> <br /> == Storage and testing ==<br /> * Leave freshly poured plates at room temperature for 3–5 days before using them. Any contamination from pouring will show up as spots or fuzz, and you can throw those plates out before they waste a culture.<br /> * Clean plates keep for weeks in the fridge, in a sealed bag, upside down.<br /> <br /> == Using plates ==<br /> * &#039;&#039;&#039;Inoculating:&#039;&#039;&#039; put a drop of spore solution or [[Tutorials/LiquidCulture/Making and Using LC|liquid culture]] onto the center, or lay a small wedge of colonized agar there.<br /> * &#039;&#039;&#039;Transfers:&#039;&#039;&#039; cut a small wedge from the leading edge of healthy growth with a flame-sterilized scalpel and move it to a fresh plate. Repeating this to clean up and select a culture is covered in [[Tutorials/Agar/Three-Transfers|the three-transfer method]].<br /> * &#039;&#039;&#039;Agar to grain:&#039;&#039;&#039; once a plate is clean and fully grown, a few wedges inoculate a jar of grain.<br /> * Incubate plates upside down at room temperature, 70–78°F.<br /> <br /> == Sources ==<br /> * Wikipedia, [https://en.wikipedia.org/wiki/Malt_extract_agar Malt extract agar]: standard composition (30 g malt extract, 20 g agar per liter)<br /> * Pouring and storage practice compiled from league members.<br /> <br /> {{Cultivation}}<br /> [[Category:Tutorials]]<br /> [[Category:Agar]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/Agar/Making_Plates https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Basics/Sterile_Technique&diff=51&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Basics/Sterile_Technique&diff=51&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Most failed grows don&#039;t fail because of a bad recipe. They fail because something else got into the jar first. Mold spores and bacteria are everywhere: on your hands, in the air, on every surface in your kitchen. Mycelium grows slowly by comparison, so anything that sneaks into a nutrient-rich jar or plate will usually win the race.<br /> <br /> Sterile technique is the set of habits that keep those passengers out during the moments your culture is exposed.<br /> <br /> == When it matters most ==<br /> Clean technique matters any time you open sterile material to the air:<br /> * Pouring and working with [[Tutorials/Agar/Making Plates|agar plates]]<br /> * Inoculating [[Tutorials/LiquidCulture/Making and Using LC|liquid culture]] or [[Tutorials/Spawn/Grain Spawn|grain jars]]<br /> * Grain-to-grain transfers<br /> <br /> It matters less when [[Tutorials/Substrate/Coir and CVG|spawning to bulk substrate]]. By then your grain is fully colonized and the mycelium can defend itself. Clean hands and a wiped-down tub are enough.<br /> <br /> == Your workspace ==<br /> === Still air box (SAB) ===<br /> A clear plastic tote turned upside down with two arm holes cut in the side. Its whole job is to stop air from moving. Spores drift on air currents, and still air lets them settle instead of landing in your jar.<br /> <br /> * Use a tote around 60–100 qt. Cut two holes about 4–5 in. wide at a comfortable reach.<br /> * Wipe the inside with 70% alcohol and let it sit a few minutes before working.<br /> * Keep your arms moving slowly. Fast motion pumps air in and out of the arm holes.<br /> * Work low in the box with jars and plates at the front, not under your arms.<br /> <br /> === Flow hood ===<br /> A flow hood blows HEPA-filtered air across your work surface, so anything floating away from you is pushed off rather than settling. It makes agar work dramatically easier, but it is a significant purchase. Work &#039;&#039;downstream&#039;&#039; of the filter: nothing should sit between the filter face and whatever you have open.<br /> <br /> === The room ===<br /> * Turn off forced-air heat, swamp coolers, and fans while you work.<br /> * Avoid rooms with houseplants, pets, or open trash.<br /> * A bathroom after running a hot shower is a classic option; the steam knocks airborne dust down.<br /> <br /> == Sanitizing ==<br /> * &#039;&#039;&#039;70% isopropyl alcohol&#039;&#039;&#039; kills more effectively than 91% or 99%. The water helps it penetrate cells, and it evaporates more slowly, giving it more contact time.<br /> * Wipe jar lids, injection ports, syringe caps, and your gloves before working.<br /> * &#039;&#039;&#039;Flame-sterilize&#039;&#039;&#039; needles and scalpels with a butane torch or alcohol lamp until the metal glows red, then let it cool a few seconds before touching culture. A hot blade will kill the tissue you&#039;re transferring.<br /> * Wear nitrile gloves and spray them often. A clean shirt, tied-back hair, and a face mask help more than they seem like they should.<br /> <br /> == Habits ==<br /> * Get everything into position before you open anything.<br /> * Open things as briefly as possible, and never reach over an open plate or jar.<br /> * Don&#039;t talk, cough, or breathe over open cultures.<br /> * Label everything immediately: what it is, which [[Tutorials/Agar/Three-Transfers|transfer]] it came from, and the date.<br /> * Keep a notebook. When something contaminates, your notes are how you figure out which step failed.<br /> <br /> == Sources ==<br /> * General technique compiled from league members&#039; practice.<br /> * North Spore, [https://northspore.com/blogs/the-black-trumpet/common-contamination-in-mushroom-cultivation Common Contamination in Mushroom Cultivation]: overview of how contaminants enter grows<br /> <br /> {{Cultivation}}<br /> [[Category:Tutorials]]<br /> [[Category:Basics]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/Basics/Sterile_Technique https://wiki.pikespeakmycoleague.org/index.php?title=Reference/Pressure_Cooking_at_Altitude&diff=50&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Reference/Pressure_Cooking_at_Altitude&diff=50&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>&#039;&#039;&#039;Short version:&#039;&#039;&#039; at Colorado Springs elevations, a pressure cooker on a 15 psi weight runs about 6°F cooler than the same cooker at sea level. Sea-level recipes that say &quot;30 minutes&quot; or &quot;90 minutes&quot; assume that extra heat. Either add pressure, if your cooker can, or add time.<br /> <br /> == Why altitude matters ==<br /> A pressure cooker&#039;s gauge or weight measures pressure &#039;&#039;above the surrounding air&#039;&#039;. The temperature inside depends on the &#039;&#039;total&#039;&#039; pressure: the air pressure outside plus what the cooker adds.<br /> <br /> At sea level the air supplies about 14.7 psi, so a 15 psi weight gives roughly 29.7 psi total and steam at about 250°F (121°C). That is the temperature lab sterilization standards are built around.<br /> <br /> At 6,000 ft the air supplies only about 11.8 psi. The same 15 psi weight gives about 26.8 psi total, and the steam only reaches about 244°F.<br /> <br /> Six degrees sounds small, but heat-resistant bacterial spores die exponentially with temperature. A common rule from sterilization science is that every 10°C (18°F) drop means you need about ten times as long for the same kill. A 3.3°C drop works out to roughly &#039;&#039;&#039;twice the time&#039;&#039;&#039; for the same level of sterility.<br /> <br /> == The numbers ==<br /> {| class=&quot;wikitable&quot;<br /> |+ Steam temperature with a 15 psi weight<br /> ! Elevation !! Air pressure !! Water boils at !! Inside cooker at 15 psi !! Gauge psi needed to match sea level<br /> |-<br /> | Sea level || 14.7 psi || 212°F || 250°F || 15.0<br /> |-<br /> | 4,000 ft || 12.7 psi || 204°F || 246°F || 17.1<br /> |-<br /> | 5,000 ft || 12.2 psi || 202°F || 245°F || 17.5<br /> |-<br /> | 6,000 ft (Colorado Springs) || 11.8 psi || 201°F || 244°F || 18.0<br /> |-<br /> | 7,000 ft (Monument) || 11.3 psi || 199°F || 243°F || 18.4<br /> |-<br /> | 8,000 ft || 10.9 psi || 197°F || 242°F || 18.8<br /> |-<br /> | 9,000 ft (above Woodland Park) || 10.5 psi || 195°F || 241°F || 19.2<br /> |}<br /> Values calculated from the standard atmosphere and water&#039;s steam table; real cookers vary a degree or two.<br /> <br /> == What to do about it ==<br /> === Option 1: add pressure (dial-gauge cookers) ===<br /> If your cooker has a dial gauge and an adjustable vent, run it at about &#039;&#039;&#039;18 psi&#039;&#039;&#039; around Colorado Springs and keep sea-level times. Check your cooker&#039;s manual for its maximum safe pressure first. Have the dial gauge tested periodically; county extension offices often do this.<br /> <br /> === Option 2: add time (weighted cookers) ===<br /> Most hobby cookers, including the popular Presto and All American models, use a weighted regulator that tops out at 15 psi. You cannot add pressure, so add time instead.<br /> <br /> {| class=&quot;wikitable&quot;<br /> |+ Suggested times at 15 psi, roughly 5,000–7,000 ft<br /> ! Item !! Common sea-level time !! Suggested here<br /> |-<br /> | Agar (up to 1 L) || 30 min || 45–60 min<br /> |-<br /> | Liquid culture jars || 30 min || 45–60 min<br /> |-<br /> | Quart jars of grain || 90 min || 120–150 min<br /> |-<br /> | 3–5 lb grain bags || 2.5 hr || 3–3.5 hr<br /> |}<br /> <br /> The doubling rule is the conservative end; sea-level times already include some safety margin, which is why many local growers get away with less. If you&#039;re seeing [[Reference/Contamination#Bacteria|bacterial contamination]] in grain, longer cook times are the first thing to try.<br /> <br /> Time starts when the weight begins to jiggle or rock steadily, not when you turn on the burner.<br /> <br /> === Overcooking ===<br /> Longer cooks darken sugars. Liquid culture and agar can turn amber and caramelize if cooked much past what they need; see the notes on [[Reference/LiquidCulture/Recipes/ClemGrows|ClemGrows LC]]. Grain is far more forgiving.<br /> <br /> == Pasteurization is different ==<br /> Bulk substrates are pasteurized, not sterilized, at about 150–170°F. Water boiling at 201°F is still well above that, so pasteurization techniques like the [[Tutorials/Substrate/Coir and CVG|bucket tek]] work fine at altitude without adjustment.<br /> <br /> == Sources ==<br /> * NMSU Cooperative Extension, [https://losalamos-extension.nmsu.edu/documents/pressure-canning-at-high-altitudes-2.pdf Pressure Canning at High Altitudes]: dial-gauge pressure increases by altitude, and why weighted gauges stay at 15 lb above 1,000 ft<br /> * Temperatures computed from the U.S. Standard Atmosphere and the Antoine equation for water<br /> <br /> {{Cultivation}}<br /> [[Category:Reference]]<br /> [[Category:Basics]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Reference/Pressure_Cooking_at_Altitude https://wiki.pikespeakmycoleague.org/index.php?title=Template:Cultivation&diff=49&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Template:Cultivation&diff=49&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>{| class=&quot;wikitable navbox&quot; style=&quot;width:100%; margin-top:2em;&quot;<br /> ! colspan=&quot;2&quot; style=&quot;text-align:center;&quot; | Cultivation guide<br /> |-<br /> ! style=&quot;text-align:right; white-space:nowrap;&quot; | Basics<br /> | [[Tutorials/Basics/Sterile Technique|Sterile technique]] · [[Reference/Pressure Cooking at Altitude|Pressure cooking at altitude]] · [[Reference/Glossary|Glossary]] · [[Resources/Equipment|Equipment]]<br /> |-<br /> ! style=&quot;text-align:right; white-space:nowrap;&quot; | Culture work<br /> | [[Tutorials/Agar/Making Plates|Agar plates]] · [[Tutorials/Agar/Three-Transfers|Three transfers]] · [[Tutorials/LiquidCulture/Making and Using LC|Liquid culture]] · LC recipes: [[Reference/LiquidCulture/Recipes/PGT|PGT]], [[Reference/LiquidCulture/Recipes/ClemGrows|ClemGrows]]<br /> |-<br /> ! style=&quot;text-align:right; white-space:nowrap;&quot; | Spawn &amp; substrate<br /> | [[Tutorials/Spawn/Grain Spawn|Grain spawn]] · [[Tutorials/Substrate/Coir and CVG|Coir and CVG]] · [[Tutorials/Substrate/Manure-Based Substrates|Manure-based substrates]]<br /> |-<br /> ! style=&quot;text-align:right; white-space:nowrap;&quot; | Fruiting &amp; problems<br /> | [[Tutorials/Fruiting/Monotub|Monotub fruiting]] · [[Reference/Contamination|Contamination ID]]<br /> |}&lt;noinclude&gt;<br /> Navigation box for the cultivation series. Add &lt;code&gt;&lt;nowiki&gt;{{Cultivation}}&lt;/nowiki&gt;&lt;/code&gt; at the bottom of any cultivation page. Styled by the &lt;code&gt;.navbox&lt;/code&gt; class.<br /> <br /> [[Category:Navigation templates]]<br /> &lt;/noinclude&gt;<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Template_talk:Cultivation https://wiki.pikespeakmycoleague.org/index.php?title=Main_Page&diff=48&oldid=43 https://wiki.pikespeakmycoleague.org/index.php?title=Main_Page&diff=48&oldid=43 <p>Wiki rebuild: cleanup and new cultivation articles</p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:00, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">== Our Founder ==</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">__NOTOC__</ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[Staff/Chris Vinci|Chris Vinci]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Welcome to the '''Pikes Peak Myco Wiki''', the knowledge base of the Pikes Peak Myco League, founded by </ins>[[Staff/Chris Vinci|Chris Vinci]]<ins style="font-weight: bold; text-decoration: none;">. It collects the techniques, recipes, and hard-won lessons of growers along the Front Range, written for people cultivating at altitude in dry Colorado air.</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">== Resources ==</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Everything here is free to use and share (CC0). If you have a recipe, a tek, or a photo of something going right or wrong, add it.</ins></div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[[Resources/Amazon|Amazon Links]]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>== <del style="font-weight: bold; text-decoration: none;">Tutorials </del>==</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>== <ins style="font-weight: bold; text-decoration: none;">New to growing? Start here </ins>==</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Read these in order. Together they take you from an empty kitchen to a first harvest.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># [[Tutorials/Basics/Sterile Technique|Sterile technique]] — why most grows fail, and how to keep yours clean</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># [[Reference/Pressure Cooking at Altitude|Pressure cooking at altitude]] — the one thing sea-level guides get wrong for us</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># [[Tutorials/Spawn/Grain Spawn|Grain spawn]] — preparing and colonizing grain jars</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># [[Tutorials/Substrate/Coir and CVG|Coir and CVG substrate]] — the bulk material your mushrooms fruit from</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># [[Tutorials/Fruiting/Monotub|Monotub fruiting]] — spawning to bulk, conditions, harvest</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"># [[Reference/Contamination|Contamination ID]] — what's growing in your tub, and what to do about it</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Culture work ==</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Agar ===</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=== Agar ===</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[Tutorials/Agar/Three-Transfers|<del style="font-weight: bold; text-decoration: none;">Three Transfer Procedure</del>]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[Tutorials/Agar/Making Plates|Making agar plates]] — recipes, pouring, storage</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* </ins>[[Tutorials/Agar/Three-Transfers|<ins style="font-weight: bold; text-decoration: none;">The three-transfer method</ins>]] <ins style="font-weight: bold; text-decoration: none;">— isolating a clean, fast genetic</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>=== Liquid <del style="font-weight: bold; text-decoration: none;">Culture </del>===</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>=== Liquid <ins style="font-weight: bold; text-decoration: none;">culture </ins>===</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[Tutorials/LiquidCulture/Making and Using LC|Making and using liquid culture]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Recipes:</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">** [[Reference/LiquidCulture/Recipes/PGT|Philly Golden Teacher LC]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">** [[Reference/LiquidCulture/Recipes/ClemGrows|ClemGrows LC]]</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>==== <del style="font-weight: bold; text-decoration: none;">Recipes ====</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>== <ins style="font-weight: bold; text-decoration: none;">Spawn and substrate </ins>==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[<del style="font-weight: bold; text-decoration: none;">Reference</del>/<del style="font-weight: bold; text-decoration: none;">LiquidCulture</del>/<del style="font-weight: bold; text-decoration: none;">Recipes</del>/<del style="font-weight: bold; text-decoration: none;">PGT</del>|<del style="font-weight: bold; text-decoration: none;">Philly Golden Teacher LC</del>]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[Tutorials/Spawn/Grain Spawn|Grain spawn]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* </ins>[[<ins style="font-weight: bold; text-decoration: none;">Tutorials/Substrate</ins>/<ins style="font-weight: bold; text-decoration: none;">Coir and CVG|Coir and CVG]] (includes the bucket tek)</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[Tutorials</ins>/<ins style="font-weight: bold; text-decoration: none;">Substrate</ins>/<ins style="font-weight: bold; text-decoration: none;">Manure-Based Substrates</ins>|<ins style="font-weight: bold; text-decoration: none;">Manure-based substrates</ins>]]</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[<del style="font-weight: bold; text-decoration: none;">https:</del>//<del style="font-weight: bold; text-decoration: none;">wiki.pikespeakmycoleague.org/index.php?title=</del>Reference/<del style="font-weight: bold; text-decoration: none;">LiquidCulture/Recipes/ClemGrows ClemGrows LC</del>]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Fruiting and troubleshooting ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* </ins>[<ins style="font-weight: bold; text-decoration: none;">[Tutorials</ins>/<ins style="font-weight: bold; text-decoration: none;">Fruiting</ins>/<ins style="font-weight: bold; text-decoration: none;">Monotub|Monotub fruiting]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[</ins>Reference/<ins style="font-weight: bold; text-decoration: none;">Contamination|Contamination ID]</ins>]</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>==<del style="font-weight: bold; text-decoration: none;">= Substrate =</del>==</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>== <ins style="font-weight: bold; text-decoration: none;">Reference </ins>==</div></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[<del style="font-weight: bold; text-decoration: none;">https:</del>//<del style="font-weight: bold; text-decoration: none;">wiki.pikespeakmycoleague.org/images/8/89</del>/<del style="font-weight: bold; text-decoration: none;">PGTCVG.jpg Bucket Tek Substrate</del>]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [</ins>[<ins style="font-weight: bold; text-decoration: none;">Reference</ins>/<ins style="font-weight: bold; text-decoration: none;">Glossary|Glossary]] — FAE, G2G, field capacity, and the rest of the jargon</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[Reference</ins>/<ins style="font-weight: bold; text-decoration: none;">Pressure Cooking at Altitude|Pressure cooking at altitude]]</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[Resources</ins>/<ins style="font-weight: bold; text-decoration: none;">Equipment|Equipment by stage]</ins>]</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Add dry ingredients to bucket (an ice chest or 5 gallon igloo work much better) boil water and add to dry ingredients, seal lid. Keep above 170 degrees for at least 2 hours to pasteurize. Makes ~11 pounds of CVG.</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Computers ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* [[Tips-And-Tricks/Computer/Linux/Shell-Commands|Linux/macOS shell command reference]]</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[https</del>://<del style="font-weight: bold; text-decoration: none;">www</del>.<del style="font-weight: bold; text-decoration: none;">youtube</del>.<del style="font-weight: bold; text-decoration: none;">com</del>/<del style="font-weight: bold; text-decoration: none;">embed</del>/<del style="font-weight: bold; text-decoration: none;">wzMRjEDMdfs?si=PyEkfVLjOfHpGKgo PGT Bucket Tek Youtube tutorial]</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">== Contributing ==</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">Anyone can edit. A few house conventions keep things tidy</ins>:</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Put tutorials under &lt;code&gt;Tutorials/&lt;</ins>/<ins style="font-weight: bold; text-decoration: none;">code&gt; and recipes or lookup tables under &lt;code&gt;Reference</ins>/<ins style="font-weight: bold; text-decoration: none;">&lt;/code&gt;</ins>.</div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Write in your own words</ins>. <ins style="font-weight: bold; text-decoration: none;">If you learned something from a video or another site, link it in a '''Sources''' section at the bottom.</ins></div></td></tr> <tr><td colspan="2" class="diff-side-deleted"></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Add &lt;code&gt;&lt;nowiki&gt;{{Cultivation}}&lt;</ins>/<ins style="font-weight: bold; text-decoration: none;">nowiki&gt;&lt;</ins>/<ins style="font-weight: bold; text-decoration: none;">code&gt; to the bottom of cultivation pages so readers can find their way around.</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[[Tutorials/Substrate/General Notes]]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">=== Computers ===</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">==== Linux/MacOS ====</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[[Tips-And-Tricks/Computer/Linux/Shell-Commands|Shell Command Reference]]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">== Useful Links ==</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* [https://www.mediawiki.org/wiki/Special:MyLanguage/Help:Contents User's Guide]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Configuration_settings Configuration settings list]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:FAQ MediaWiki FAQ]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* [https://lists.wikimedia.org/postorius/lists/mediawiki-announce.lists.wikimedia.org/ MediaWiki release mailing list]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* [https://www.mediawiki.org/wiki/Special:MyLanguage/Localisation#Translation_resources Localise MediaWiki for your language]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Combating_spam Learn how to combat spam on your wiki]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> </table> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Main_Page https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Substrate/Coir_and_CVG&diff=58&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Substrate/Coir_and_CVG&diff=58&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>&#039;&#039;&#039;Bulk substrate&#039;&#039;&#039; is the material your mushrooms actually fruit from. It&#039;s less nutritious than grain on purpose: it gives the mycelium water and structure while making it harder for contaminants to compete. Because of that, bulk only needs to be &#039;&#039;&#039;pasteurized&#039;&#039;&#039;, which kills most competitors while leaving harmless microbes that help defend it.<br /> <br /> Coir-based substrates are the easiest place to start. They&#039;re cheap, clean, consistent, and hard to mess up.<br /> <br /> == Ingredients ==<br /> * &#039;&#039;&#039;Coco coir:&#039;&#039;&#039; shredded coconut husk, sold as compressed bricks (often 650 g). It holds water well and resists contamination.<br /> * &#039;&#039;&#039;Vermiculite:&#039;&#039;&#039; a puffed mineral that holds water and keeps the mix airy. Use horticultural grade.<br /> * &#039;&#039;&#039;Gypsum:&#039;&#039;&#039; calcium sulfate. Adds calcium and sulfur and helps keep the mix from compacting.<br /> <br /> &#039;&#039;&#039;CVG&#039;&#039;&#039; stands for &#039;&#039;&#039;C&#039;&#039;&#039;oir, &#039;&#039;&#039;V&#039;&#039;&#039;ermiculite, &#039;&#039;&#039;G&#039;&#039;&#039;ypsum.<br /> <br /> == Recipes ==<br /> [[File:PGTCVG.jpg|thumb|right|Recipe card for coir and CVG, from Philly Golden Teacher]]<br /> {| class=&quot;wikitable&quot;<br /> ! Recipe !! Coir !! Vermiculite !! Gypsum !! Water<br /> |-<br /> | &#039;&#039;&#039;Coir only&#039;&#039;&#039; || 1 brick (650 g) || – || – || 3.5 qt (14 cups)<br /> |-<br /> | &#039;&#039;&#039;CVG&#039;&#039;&#039; || 1 brick (650 g) || 2 qt (8 cups) || 1 cup || 4.5 qt (18 cups)<br /> |}<br /> One brick of CVG makes about &#039;&#039;&#039;11 lb&#039;&#039;&#039; of hydrated substrate, enough for a typical 54–66 qt [[Tutorials/Fruiting/Monotub|monotub]] with 2–3 quarts of spawn.<br /> <br /> These water amounts are for &#039;&#039;&#039;dry, compressed bricks&#039;&#039;&#039;. Loose bags of &quot;coco coir potting mix&quot; often come partly hydrated already. Start with roughly half the water and add more only if needed.<br /> <br /> == Bucket tek: pasteurize and hydrate in one step ==<br /> # Break up the coir brick and add it with the vermiculite and gypsum to a 5-gallon bucket with a lid. An insulated cooler or 5-gallon Igloo drink cooler works even better because it holds heat longer.<br /> # Bring the water to a full boil and pour it over the dry ingredients.<br /> # Seal the lid immediately.<br /> # Leave it closed. The goal is to keep it &#039;&#039;&#039;above about 150–170°F for at least 2 hours&#039;&#039;&#039;. A cooler will do this on its own; a plain bucket can be wrapped in towels or a blanket.<br /> # Let it sit until it&#039;s cooled to room temperature, usually overnight. &#039;&#039;&#039;Give it at least 6 hours&#039;&#039;&#039; so the coir fully absorbs the water.<br /> # Break up any clumps with clean, gloved hands before mixing with spawn.<br /> <br /> At Colorado Springs elevations water boils at about 201°F instead of 212°F, which is still well above pasteurization temperature. The bucket tek doesn&#039;t need any altitude adjustment.<br /> <br /> == Field capacity ==<br /> Substrate should be at &#039;&#039;&#039;field capacity&#039;&#039;&#039;: holding as much water as it can without any pooling. The test:<br /> * Grab a handful and squeeze hard.<br /> * &#039;&#039;&#039;Just right:&#039;&#039;&#039; a few drops run out, and it holds together loosely when you open your hand.<br /> * &#039;&#039;&#039;Too wet:&#039;&#039;&#039; a stream of water runs out. Excess water invites [[Reference/Contamination#Bacteria|bacteria]].<br /> * &#039;&#039;&#039;Too dry:&#039;&#039;&#039; nothing comes out and it crumbles. Mushrooms are mostly water, so dry substrate means small flushes.<br /> <br /> === Fixing soupy substrate ===<br /> If the mix still looks like soup a couple of hours in:<br /> * Stir in about 2 cups of dry coir and 1 cup of vermiculite, wait 30 minutes, and check again.<br /> * Keeping it hot (a pot on low heat, steaming) speeds absorption and drives off a bit of the extra water.<br /> * Draining it through a clean pillowcase or mesh bag and wringing it out also works.<br /> <br /> == Spawning ==<br /> Mix colonized [[Tutorials/Spawn/Grain Spawn|grain spawn]] into the substrate at somewhere between &#039;&#039;&#039;1:1 and 1:4 spawn to substrate&#039;&#039;&#039; by volume. More spawn colonizes faster and resists contamination better. Then follow [[Tutorials/Fruiting/Monotub|monotub fruiting]].<br /> <br /> == Sources ==<br /> * Philly Golden Teacher, [https://www.youtube.com/watch?v=wzMRjEDMdfs Bucket tek substrate video] (YouTube): the recipe card above<br /> * Notes on loose coir, soaking time, and fixing over-wet substrate contributed by league members.<br /> <br /> {{Cultivation}}<br /> [[Category:Tutorials]]<br /> [[Category:Substrate]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/Substrate/Coir_and_CVG https://wiki.pikespeakmycoleague.org/index.php?title=Category:Navigation_templates&diff=79&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Navigation_templates&diff=79&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Templates used for navigating the wiki.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Navigation_templates https://wiki.pikespeakmycoleague.org/index.php?title=Category:Computers&diff=78&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Computers&diff=78&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Computer tips for growers.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Computers https://wiki.pikespeakmycoleague.org/index.php?title=Category:Resources&diff=77&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Resources&diff=77&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Equipment, suppliers, and outside links.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Resources https://wiki.pikespeakmycoleague.org/index.php?title=Category:Contamination&diff=76&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Contamination&diff=76&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Identifying and dealing with contamination.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Contamination https://wiki.pikespeakmycoleague.org/index.php?title=Category:Fruiting&diff=75&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Fruiting&diff=75&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Getting from colonized substrate to harvest.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Fruiting https://wiki.pikespeakmycoleague.org/index.php?title=Category:Substrate&diff=74&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Substrate&diff=74&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Bulk substrates and how to prepare them.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Substrate https://wiki.pikespeakmycoleague.org/index.php?title=Category:Spawn&diff=73&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Spawn&diff=73&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Grain spawn and spawn production.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Spawn https://wiki.pikespeakmycoleague.org/index.php?title=Category:Liquid_culture&diff=72&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Liquid_culture&diff=72&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Making and using liquid culture.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Liquid_culture https://wiki.pikespeakmycoleague.org/index.php?title=Category:Agar&diff=71&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Agar&diff=71&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Working with agar plates.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Agar https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Substrate/Manure-Based_Substrates&diff=59&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Substrate/Manure-Based_Substrates&diff=59&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Many cultivated mushrooms are &#039;&#039;&#039;coprophilic&#039;&#039;&#039;: in the wild they grow on dung, mostly from grazing animals. They&#039;ll fruit happily on plain [[Tutorials/Substrate/Coir and CVG|CVG]], but adding manure gives them the food they evolved on. Growers commonly report heavier, more consistent flushes, at the cost of more prep and more contamination risk.<br /> <br /> If you&#039;re new, get a couple of grows done on CVG first. Manure is a step up.<br /> <br /> == Choosing manure ==<br /> * &#039;&#039;&#039;Horse manure&#039;&#039;&#039; is the standard. Horses digest grass less efficiently than cattle, so their manure keeps more usable fiber. Cow manure also works.<br /> * &#039;&#039;&#039;Aged or composted&#039;&#039;&#039; manure is what you want: dark, crumbly, earthy-smelling, with no ammonia smell. Fresh manure is too hot, too wet, and full of competitors.<br /> * &#039;&#039;&#039;Ask about dewormers.&#039;&#039;&#039; Many growers avoid manure from horses recently treated with dewormers, since the residues pass through into the manure.<br /> * &#039;&#039;&#039;Bagged composted manure&#039;&#039;&#039; from garden centers is convenient. Read the label and skip anything with added fertilizer, wetting agents, or &quot;moisture control&quot; additives.<br /> * Around the Pikes Peak region, stables and horse properties are often happy to give away aged manure. Bring your own bags and pick the oldest pile.<br /> <br /> Dry it before storing. Sun-dried manure keeps indefinitely and is much less smelly to handle.<br /> <br /> == Recipes ==<br /> Ratios are by volume. All of these take a little gypsum, about 1 cup per 5 gallons of finished mix.<br /> <br /> {| class=&quot;wikitable&quot;<br /> ! Mix !! Manure !! Coir !! Vermiculite !! Notes<br /> |-<br /> | &#039;&#039;&#039;50/50&#039;&#039;&#039; || 1 part || 1 part || – || The most common starting point<br /> |-<br /> | &#039;&#039;&#039;Manure CVG&#039;&#039;&#039; || 1 part || 1 part || 1 part || Lighter and airier, holds water well<br /> |-<br /> | &#039;&#039;&#039;Manure-heavy&#039;&#039;&#039; || 2 parts || 1 part || – || Richest. Highest contamination risk.<br /> |}<br /> <br /> Break up or sift the dried manure so there are no big chunks, then mix it dry with the other ingredients before adding water.<br /> <br /> == Pasteurizing ==<br /> Manure carries far more competitors than coir, so pasteurization matters more. The target is &#039;&#039;&#039;150–170°F for 1–2 hours&#039;&#039;&#039;. Going much hotter starts to kill off the beneficial microbes too.<br /> <br /> === Bucket or cooler ===<br /> The same [[Tutorials/Substrate/Coir and CVG#Bucket tek: pasteurize and hydrate in one step|bucket tek]] as CVG: dry mix in a cooler, boiling water poured over, lid sealed. Use a thermometer the first few times to confirm it holds temperature; denser manure mixes cool faster than plain coir.<br /> <br /> === Oven or stovetop ===<br /> # Hydrate the mix to [[Tutorials/Substrate/Coir and CVG#Field capacity|field capacity]] first.<br /> # Pack it into an oven bag or a covered pot.<br /> # Hold at 160–170°F for 1–2 hours. Use a probe thermometer in the center of the mass; the oven setting will be higher than the substrate temperature.<br /> # Let it cool, covered, to room temperature.<br /> <br /> === Hydration ===<br /> Manure absorbs water differently than coir. Add water gradually and do the squeeze test as you go. Aim for the same field capacity: a few drops when squeezed hard.<br /> <br /> == Working with it ==<br /> * Spawn at 1:1 to 1:2 by volume. A heavier spawn rate helps the mycelium claim the substrate before anything else can.<br /> * Expect a slightly longer colonization than CVG.<br /> * Many growers top manure mixes with a thin &#039;&#039;&#039;casing layer&#039;&#039;&#039; of plain hydrated coir or CVG. It keeps the surface cleaner and holds moisture for pinning.<br /> * It smells. Keep tubs somewhere with good ventilation.<br /> <br /> == Troubleshooting ==<br /> * &#039;&#039;&#039;Ammonia smell after pasteurization:&#039;&#039;&#039; the manure wasn&#039;t aged enough. Ammonia inhibits mycelium; start over with older manure.<br /> * &#039;&#039;&#039;Green or other colored mold in the first week:&#039;&#039;&#039; pasteurization was too short or too cool. See [[Reference/Contamination|contamination ID]].<br /> * &#039;&#039;&#039;Slow, patchy colonization:&#039;&#039;&#039; too wet, or too little spawn.<br /> <br /> {{Cultivation}}<br /> [[Category:Tutorials]]<br /> [[Category:Substrate]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/Substrate/Manure-Based_Substrates https://wiki.pikespeakmycoleague.org/index.php?title=Category:Recipes&diff=69&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Recipes&diff=69&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Tested recipes for agar, liquid culture, and substrate.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Recipes https://wiki.pikespeakmycoleague.org/index.php?title=Category:Reference&diff=68&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Reference&diff=68&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Lookup tables, recipes, and definitions.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Reference https://wiki.pikespeakmycoleague.org/index.php?title=Category:Tutorials&diff=67&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Category:Tutorials&diff=67&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Step-by-step guides.<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Category_talk:Tutorials https://wiki.pikespeakmycoleague.org/index.php?title=Tips-And-Tricks/Computer/Linux/Shell-Commands&diff=66&oldid=39 https://wiki.pikespeakmycoleague.org/index.php?title=Tips-And-Tricks/Computer/Linux/Shell-Commands&diff=66&oldid=39 <p>Wiki rebuild: cleanup and new cultivation articles</p> <a href="https://wiki.pikespeakmycoleague.org/index.php?title=Tips-And-Tricks/Computer/Linux/Shell-Commands&amp;diff=66&amp;oldid=39">Show changes</a> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tips-And-Tricks/Computer/Linux/Shell-Commands https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Substrate/General_Notes&diff=65&oldid=40 https://wiki.pikespeakmycoleague.org/index.php?title=Tutorials/Substrate/General_Notes&diff=65&oldid=40 <p>Wiki rebuild: cleanup and new cultivation articles</p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:00, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">== Some Notes from a first time making Sub ==</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">#REDIRECT [[Tutorials/Substrate/Coir and CVG]]</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">=== This applies to making standard CVG substrate only, other types differ ===</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* The values for the amount of water to add is based on dried, compressed bricks of CVG. If you happen to get a bag of generic "Coco coir potting mix", it has most of that already in it. Reduce the amount of water by roughly half.</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* Keep the sub warm for several hours and give it at least 6 hours to soak up all the water.</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">* If you accidentally put too much water in and its "soup" after about 2 hours, add 2 cups of coir and 1 cup of vermiculite then stir, let sit for 30 mins and reevaluate. Also, put it on low heat to keep it really warm and steaming. This will speed up absorption and help evaporate a little of the excess.</del></div></td><td colspan="2" class="diff-side-added"></td></tr> </table> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Tutorials/Substrate/General_Notes https://wiki.pikespeakmycoleague.org/index.php?title=Resources/Amazon&diff=64&oldid=22 https://wiki.pikespeakmycoleague.org/index.php?title=Resources/Amazon&diff=64&oldid=22 <p>Wiki rebuild: cleanup and new cultivation articles</p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 19:00, 22 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">Anyone can do a basic search on Amazon, but these are some of the harder </del>to find items <del style="font-weight: bold; text-decoration: none;">and their usage</del></div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">#REDIRECT [[Resources/Equipment#Hard-</ins>to<ins style="font-weight: bold; text-decoration: none;">-</ins>find items<ins style="font-weight: bold; text-decoration: none;">]]</ins></div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">=== Bag Tek ===</del></div></td><td colspan="2" class="diff-side-added"></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">[https://a.co/d/0gaNVxrY Clear Thick Tape to hold injection ports in place]</del></div></td><td colspan="2" class="diff-side-added"></td></tr> </table> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Resources/Amazon https://wiki.pikespeakmycoleague.org/index.php?title=Resources/Equipment&diff=63&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Resources/Equipment&diff=63&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>What you need, organized by stage. You don&#039;t need all of it to start; the &#039;&#039;&#039;Essentials&#039;&#039;&#039; column is enough for a first monotub grow from a spore syringe or liquid culture.<br /> <br /> Anyone can do a basic search on Amazon. Where an item is genuinely hard to find, it&#039;s linked under [[#Hard-to-find items|hard-to-find items]] below.<br /> <br /> == By stage ==<br /> {| class=&quot;wikitable&quot;<br /> ! Stage !! Essentials !! Nice to have<br /> |-<br /> | &#039;&#039;&#039;Clean work&#039;&#039;&#039; || Still air box (a 60–100 qt clear tote), 70% isopropyl alcohol in a spray bottle, nitrile gloves, face mask || Flow hood, butane torch, alcohol lamp<br /> |-<br /> | &#039;&#039;&#039;Sterilizing&#039;&#039;&#039; || Pressure cooker that holds 15 psi (a 16–23 qt model fits quart jars) || A larger canner or an All American for bigger batches; a dial gauge for [[Reference/Pressure Cooking at Altitude|altitude adjustment]]<br /> |-<br /> | &#039;&#039;&#039;Agar&#039;&#039;&#039; || Petri dishes, agar powder, light malt extract, scalpel || Media bottles, Parafilm, a digital scale that reads to 0.1 g<br /> |-<br /> | &#039;&#039;&#039;Liquid culture&#039;&#039;&#039; || Mason jars, lids with injection ports and filters, syringes with 16–18 gauge needles || Magnetic stir plate and stir bars<br /> |-<br /> | &#039;&#039;&#039;Grain spawn&#039;&#039;&#039; || Quart mason jars or filter-patch grain bags, lids with filters, grain (oats, rye, or millet) || Impulse sealer for bags<br /> |-<br /> | &#039;&#039;&#039;Bulk substrate&#039;&#039;&#039; || Coco coir bricks, vermiculite, gypsum, 5-gallon bucket or cooler with lid || Probe thermometer<br /> |-<br /> | &#039;&#039;&#039;Fruiting&#039;&#039;&#039; || 54–66 qt tote, micropore tape or polyfill, spray mister || Hygrometer, clip-on LED, fan<br /> |-<br /> | &#039;&#039;&#039;After harvest&#039;&#039;&#039; || Paper towels, clean containers || Food dehydrator<br /> |}<br /> <br /> == Hard-to-find items ==<br /> === Bag tek ===<br /> * [https://a.co/d/0gaNVxrY Clear thick tape] for holding injection ports in place on grain bags<br /> <br /> &#039;&#039;Add more here as you find them. Include what it&#039;s for, not just the link.&#039;&#039;<br /> <br /> == Local suppliers ==<br /> &#039;&#039;List Colorado or Front Range shops that sell cultivation supplies, with what they&#039;re good for.&#039;&#039;<br /> <br /> {{Cultivation}}<br /> [[Category:Resources]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Resources/Equipment https://wiki.pikespeakmycoleague.org/index.php?title=Reference/Glossary&diff=62&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Reference/Glossary&diff=62&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Common terms and abbreviations used by growers and on this wiki.<br /> <br /> ; Aborts : Pins that stop growing, usually turning dark or shriveled. Typically caused by the surface drying out.<br /> ; Agar : A seaweed-derived gel used as a growing surface in petri dishes. See [[Tutorials/Agar/Making Plates|making agar plates]].<br /> ; BRF : Brown rice flour. Used in the classic &quot;PF tek&quot; cake method.<br /> ; Bulk : Bulk substrate. The large mass of pasteurized material that spawn is mixed into for fruiting.<br /> ; Casing layer : A thin layer of moist, low-nutrient material placed on top of colonized substrate to hold moisture and encourage pinning.<br /> ; Coir : Shredded coconut husk. The base of most bulk substrates.<br /> ; Colonization : The mycelium growing through and claiming a material.<br /> ; Contam : Contamination. Any unwanted mold, bacteria, or yeast. See [[Reference/Contamination|contamination ID]].<br /> ; Coprophilic : Dung-loving. Describes species that grow on manure in nature. See [[Tutorials/Substrate/Manure-Based Substrates|manure-based substrates]].<br /> ; CVG : Coir, vermiculite, gypsum. The most common bulk substrate. See [[Tutorials/Substrate/Coir and CVG|coir and CVG]].<br /> ; Dunk : Soaking a spent cake or tub in water between flushes to rehydrate it.<br /> ; Exudate : Yellowish liquid droplets pushed out by mycelium. Not contamination. Also called metabolites.<br /> ; FAE : Fresh air exchange. Replacing CO₂-rich air with fresh air, which triggers pinning.<br /> ; Field capacity : The maximum water a substrate holds without pooling. A few drops when squeezed hard.<br /> ; Flow hood : A fan blowing air through a HEPA filter to create a clean work area.<br /> ; Flush : One wave of mushrooms. A tub usually produces several flushes.<br /> ; G2G : Grain to grain. Using colonized grain to inoculate fresh sterile grain.<br /> ; Gypsum : Calcium sulfate. Added to substrates and grain for calcium and structure.<br /> ; Hyphae : The individual thread-like cells that make up mycelium.<br /> ; Inoculation : Introducing spores or mycelium into a sterile or pasteurized medium.<br /> ; Isolate : A culture selected and cleaned up to a single genetic line, usually on agar.<br /> ; LC : Liquid culture. Mycelium grown in sugar water. See [[Tutorials/LiquidCulture/Making and Using LC|making and using LC]].<br /> ; LME : Light malt extract. A common sugar in agar and LC.<br /> ; Monotub : A storage tote modified into a fruiting chamber. See [[Tutorials/Fruiting/Monotub|monotub fruiting]].<br /> ; MS : Multispore. A syringe or print containing spores from many individuals.<br /> ; Mycelium : The vegetative body of a fungus; the white network of threads you grow before mushrooms appear.<br /> ; Pasteurization : Heating to roughly 150–170°F to kill most competitors while leaving some beneficial microbes. Used for bulk substrate.<br /> ; PC : Pressure cooker. See [[Reference/Pressure Cooking at Altitude|pressure cooking at altitude]].<br /> ; Pins : Baby mushrooms. Also called primordia.<br /> ; Rhizomorphic : Ropey, root-like mycelium. Often a sign of vigorous genetics.<br /> ; SAB : Still air box. A clear tote with arm holes for clean work. See [[Tutorials/Basics/Sterile Technique|sterile technique]].<br /> ; Sector : A visibly distinct region of growth on agar, usually a different genetic individual.<br /> ; Spawn : A nutritious material, usually grain, fully colonized by mycelium and used to inoculate bulk.<br /> ; Spawn ratio : The ratio of spawn to bulk substrate, by volume. 1:2 means one part spawn to two parts substrate.<br /> ; Spore print : Spores collected by letting a mature cap drop them onto paper or foil.<br /> ; Sterilization : Killing everything, normally with a pressure cooker at 15 psi. Required for grain, agar, and LC.<br /> ; Substrate : Any material mycelium grows on and feeds from.<br /> ; T1, T2, T3 : Transfer generation on agar. See [[Tutorials/Agar/Three-Transfers|the three-transfer method]].<br /> ; Tek : A technique or method.<br /> ; Tomentose : Fluffy, cottony mycelium without distinct strands.<br /> ; Trich : &#039;&#039;Trichoderma&#039;&#039;, a common green mold.<br /> ; Veil : A thin membrane covering the gills of young mushrooms. Tearing usually signals harvest time.<br /> ; Vermiculite : A puffed mineral that holds water and keeps substrate airy.<br /> ; WBS : Wild bird seed, used as grain spawn.<br /> <br /> {{Cultivation}}<br /> [[Category:Reference]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Reference/Glossary https://wiki.pikespeakmycoleague.org/index.php?title=Reference/Contamination&diff=61&oldid=0 https://wiki.pikespeakmycoleague.org/index.php?title=Reference/Contamination&diff=61&oldid=0 <p>Wiki rebuild: cleanup and new cultivation articles</p> <p><b>New page</b></p><div>Every grower deals with contamination eventually. This page helps you work out what you&#039;re looking at and whether anything can be saved.<br /> <br /> &#039;&#039;&#039;When in doubt, throw it out.&#039;&#039;&#039; A doubtful jar is rarely worth risking the rest of your grow over. And &#039;&#039;&#039;never open a contaminated container indoors&#039;&#039;&#039;. Bag it sealed and dispose of it outside; opening it releases millions of spores into the room where you do your clean work.<br /> <br /> == Not contamination ==<br /> Plenty of normal things look alarming the first time. Check these before throwing anything out.<br /> <br /> {| class=&quot;wikitable&quot;<br /> ! What you see !! What it is<br /> |-<br /> | &#039;&#039;&#039;Yellow or amber droplets&#039;&#039;&#039; on the mycelium, no smell || &#039;&#039;&#039;Metabolites&#039;&#039;&#039; (exudate). The mycelium is pushing out excess water and byproducts. Often a sign of stress, but not contamination.<br /> |-<br /> | &#039;&#039;&#039;Blue or bluish-green staining&#039;&#039;&#039; where mycelium touches the jar or tub, or where it&#039;s been handled || &#039;&#039;&#039;Bruising&#039;&#039;&#039; in species that stain blue. Test: rub it gently with a cotton swab. Mold leaves powder on the swab; bruising doesn&#039;t.<br /> |-<br /> | &#039;&#039;&#039;Fluffy, cottony white patches&#039;&#039;&#039; || &#039;&#039;&#039;Tomentose&#039;&#039;&#039; growth or &#039;&#039;&#039;aerial mycelium&#039;&#039;&#039;, usually from high humidity or CO₂. Still white and still yours.<br /> |-<br /> | &#039;&#039;&#039;Dense white knots&#039;&#039;&#039; || &#039;&#039;&#039;Hyphal knots&#039;&#039;&#039;: the very first stage of pins.<br /> |}<br /> <br /> == Molds ==<br /> === Trichoderma (green mold) ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; starts as fast, dense, bright white growth, then turns emerald or forest green, usually in a ring or patch.<br /> * &#039;&#039;&#039;Where:&#039;&#039;&#039; bulk substrate, grain, agar. The most common serious contaminant.<br /> * &#039;&#039;&#039;Cause:&#039;&#039;&#039; its spores are in almost every soil. They get in through poor sterile technique, under-pasteurized substrate, or open air.<br /> * &#039;&#039;&#039;What to do:&#039;&#039;&#039; toss it. Trich spreads quickly and sporulates heavily. On agar, it can sometimes be outrun by transferring clean mycelium far away from it.<br /> <br /> === Cobweb mold ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; gray-white, very fine and wispy, like spider web. It floats &#039;&#039;above&#039;&#039; the surface instead of growing in it, and spreads extremely fast, sometimes across a whole tub in a day or two. Often appears late in colonization or during fruiting.<br /> * &#039;&#039;&#039;Versus mycelium:&#039;&#039;&#039; mycelium is denser and ropier and hugs the substrate.<br /> * &#039;&#039;&#039;Cause:&#039;&#039;&#039; stagnant, humid air and low fresh air exchange.<br /> * &#039;&#039;&#039;What to do:&#039;&#039;&#039; if caught early, spray the patch with 3% hydrogen peroxide. Then improve fresh air in the tub. If it has spread or come back, toss it.<br /> <br /> === Penicillium ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; blue-green or gray-green powdery spots, often with a white rim.<br /> * &#039;&#039;&#039;Where:&#039;&#039;&#039; common on agar and grain.<br /> * &#039;&#039;&#039;What to do:&#039;&#039;&#039; toss grain; transfer away from it on agar.<br /> <br /> === Aspergillus ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; fuzzy black, yellow-green, or olive patches with a powdery, sometimes granular surface.<br /> * &#039;&#039;&#039;Caution:&#039;&#039;&#039; some &#039;&#039;Aspergillus&#039;&#039; species can cause lung infections in people with weakened immune systems. Bag it without opening it, and wear a mask while handling it.<br /> <br /> === Black pin mold (&#039;&#039;Rhizopus&#039;&#039;) ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; tall gray wisps topped with tiny black dots, like pins in a pincushion.<br /> * &#039;&#039;&#039;What to do:&#039;&#039;&#039; toss.<br /> <br /> === Neurospora (orange bread mold) ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; wispy white turning bright orange and powdery.<br /> * &#039;&#039;&#039;Caution:&#039;&#039;&#039; one of the fastest-spreading molds there is. It can colonize in hours and is very hard to get rid of once it&#039;s in your workspace. Seal and dispose of it immediately.<br /> <br /> === Lipstick mold ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; white patches that turn pinkish-red, usually on the surface of bulk or casing layers.<br /> * &#039;&#039;&#039;What to do:&#039;&#039;&#039; remove it if small, otherwise toss.<br /> <br /> == Bacteria ==<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; wet, slimy, or greasy patches, often gray, tan, or yellowish. In grain jars it shows up as kernels that look shiny, wet, or milky, often at the bottom of the jar, with the mycelium refusing to grow into that area. In liquid culture, the whole liquid turns cloudy.<br /> * &#039;&#039;&#039;Smell:&#039;&#039;&#039; sour, like old socks or rotten fruit. Smell is often the quickest test.<br /> * &#039;&#039;&#039;Cause:&#039;&#039;&#039; almost always &#039;&#039;&#039;too much water&#039;&#039;&#039;. Wet grain, soupy substrate, or standing water in jars. Bacterial endospores also survive short pressure cooks, which is why grain should be soaked first and [[Reference/Pressure Cooking at Altitude|cooked long enough for your altitude]].<br /> * &#039;&#039;&#039;What to do:&#039;&#039;&#039; toss grain and LC. In a bulk tub, a small patch can be scooped out with a sterilized spoon. Fix the moisture problem for next time.<br /> <br /> === Bacterial blotch ===<br /> * &#039;&#039;&#039;Looks like:&#039;&#039;&#039; yellow-brown, slightly sunken, slimy spots on the caps or stems of growing mushrooms.<br /> * &#039;&#039;&#039;Cause:&#039;&#039;&#039; water sitting on the mushrooms. Usually from misting fruits directly, or poor air movement.<br /> * &#039;&#039;&#039;What to do:&#039;&#039;&#039; remove affected mushrooms. Mist the tub walls, not the mushrooms, and increase fresh air.<br /> <br /> == Pests ==<br /> * &#039;&#039;&#039;Fungus gnats:&#039;&#039;&#039; tiny black flies around tubs. Their larvae tunnel through substrate and mushrooms. Keep filters intact and use sticky traps nearby.<br /> * &#039;&#039;&#039;Mites:&#039;&#039;&#039; tiny moving specks, usually from old substrate or contaminated supplies. Keep work areas clean and keep old grows away from new ones.<br /> <br /> == Sources ==<br /> * North Spore, [https://northspore.com/blogs/the-black-trumpet/common-contamination-in-mushroom-cultivation Common Contamination in Mushroom Cultivation]: descriptions of Trichoderma, cobweb, Neurospora, Rhizopus and bacterial contamination; hydrogen peroxide treatment for cobweb; the swab test for bruising<br /> * Additional identification notes compiled from league members.<br /> <br /> {{Cultivation}}<br /> [[Category:Reference]]<br /> [[Category:Contamination]]<br /> </div> Thu, 22 Oct 2026 19:00:00 GMT imported>Claude https://wiki.pikespeakmycoleague.org/index.php?title=Talk:Reference/Contamination